Regulatory

Part:BBa_K188727

Designed by: Hsiao-Yun Ko   Group: iGEM09_NCTU_Formosa   (2009-10-19)


TetR repressible promoter ( weak )

Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. This modified part use Degenerated PCR to make different TATA box at -10 sequences with original part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None